This experiment was carried out to establish a proper method of indocyanine green(ICG) excretion test for a applicable liver function test in dogs.
The half life(Tl/2), fractional clearance rate(KICG) and retention rate after injection of ICG with or without administered carbon tetrachloride(CCl₄) were also invested.
The results obtained were as follows ;
1. The maximum absorbance of ICG in plasma was at 810㎚.
2. Half life and fractional clearance rate when administered 0.25 and 0.50㎎ of ICG per Kilogram body weight were 6.33 t 0.58 minutes and 0.11±0.99/minute in the former, 10.01±1.0 minutes and 0.07±0.007/minute in the latter, respectively.
The ICG removal rate was exponentially linear for the first 15 minutes after injection both, of 0.25 and 0.50㎎ of ICG.
3. One day following the administration of 0.0042㎖ CCl₄ kilogram body weight which injected 0.50㎎ of ICG, half life was more longer and fractional clearance rate was significantly reduced than that of ICG single injection.
4. Plasma retention rate when 15, 30, 45 minutes after injection dose of 0.25 and 0.50㎎ ICG per Kilogram body weight, 14.7±4.8, 5.1±3.1, 2.6±1.6% in the former, 26.9±1.8, 11.1±2.4, 4.8±1.3% in the latter, respectively.
However, after administration of CCI₄, plasma retention rate of ICG at a dose of 0.50㎎, it was 39.3±0.9, 16±2.9, 10.7±0.1%, respectively.
5. Plasma enzyme(AST, ALT, r-GTP) activities administered with CCI₄ were increased, but there was no change which injected any dose of single ICG injection.
From these results, ICG excretion test to dog is applicable to evaluation of liver function in both clinical and research.
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